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How to make agar

  • Aug 10
  • 1 min read
  1. Start with a standard recipe of


    1L Water (distilled preferable)


    20g Agar


    14.5g LME


    1g yeast (optional)


  2. Combine Ingredients

    Place all dry ingredients into a small plastic bowl.


  3. Heat Water

    Boil 1 liter of water.


  4. Prepare Media Bottle

    Drop a stir bar into the media bottle.

    Place the media bottle on the stir plate.


  5. Add Initial Water

    Using a stainless steel funnel, carefully pour half of the boiling water into the media bottle.

    Turn on the stir plate to create strong water movement.


  6. Add Ingredients

    Transfer the dry ingredients from the bowl into the media bottle through the funnel.

    Ensure the stir bar continues spinning and is not disturbed.


  7. Collect Residual Ingredients

    Add a small amount of hot water to the bowl, swirl to gather any remaining ingredients, and pour into the media bottle.


  8. Fill to Capacity

    Add the remaining boiling water until the media bottle reaches the desired volume.

    (Optional) Add food coloring for visual reference.


  9. Mix Thoroughly

    Allow the stir plate to run for several minutes, or until no visible sediment remains.


  10. Prepare for Sterilization

    Place the lid on the media bottle, finger-tight.

    Securely cover the lid with aluminum foil.


  11. Sterilize

    Pour 3L of water into the pressure cooker.

    Place the media bottle into the pressure cooker on top of some raised platform / trivet so the water isn’t touching the bottles.

    Bring the cooker to 15 PSI and maintain the required sterilization time.

    Let the pressure cook pressure cook 25-30 minutes.

    Once that is done, allow the pressure cooker to depressurize naturally.

If you do not want to dive into making agar.


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