Tutorials

Supplies https://linktr.ee/MushysMarket How to Make Substrate (Bucket Tek) Ingredients 4 quarts water (1 gallon) 1 coco coir brick (650 g) 2 quarts vermiculite 1/2 cup gypsum 1. Prepare the Coir Bring 4 quarts of water to a boil. Place the 650 g coco coir brick into a clean bucket. Add 2 quarts of vermiculite. Add 1/2 cup of gypsum Pour the water in the bucket and allow it get all over the ingredients. The coir will expand. 2. Mix it up Use a drill with a mixing attachment (o

Supplies https://linktr.ee/MushysMarket How to Pour Agar Plates (Step-by-Step) 1. Cool the Agar After sterilization, remove the agar container from the pressure cooker. Allow it to cool to about 125°F (52°C) Use a temperature gun to monitor. If it cools too much, the agar will solidify before pouring. While cooling, place the container on a stir plate to keep the agar spinning. This prevents settling or clumping. 2. Prepare Your Workspace Set up in front of a flow hood or ins

Supplies https://linktr.ee/MushysMarket Start with a standard recipe of 1L Water (distilled preferable) 20g Agar 14.5g LME 1g yeast (optional) Combine Ingredients Place all dry ingredients into a small plastic bowl. Heat Water Boil 1 liter of water. Prepare Media Bottle Drop a stir bar into the media bottle. Place the media bottle on the stir plate. Add Initial Water Using a stainless steel funnel, carefully pour half of the boiling water into the media bottle. Turn on the st

Supplies links below https://linktr.ee/MushysMarket 1. Prepare Modified Lids Gather your jar lids. Drill a ¼-inch hole for the filter. (Optional) Drill a second ¼-inch hole if you want a liquid culture (LC) injection port. Placement doesn’t matter; anywhere on the lid is fine. Attach your filter (and LC port, if added). 2. Measure Grain and Water Use a scale to weigh out 250 g of millet per jar. Measure 125 g of water separately. (1 g ≈ 1 mL) Do not measure water directly in

This covers: Swab to agar Vial to agar Lc to agar Agar to grain Lc to grain How to make a dubtub